Leukotriene A4 Hydrolase/LTA4H Protein, Human (HEK293, His)

The leukotriene A4 hydrolase/LTA4H protein is a bifunctional zinc metalloenzyme that functions as an epoxide hydrolase to convert LTA4 to the proinflammatory mediator LTB4 and exhibits aminopeptidase activity targeting the N-terminal arginine. In addition to its pro-inflammatory effects, LTA4H also combats inflammation by inactivating the neutrophil attractant Pro-Gly-Pro. Leukotriene A4 Hydrolase/LTA4H Protein, Human (HEK293, His) is the recombinant human-derived Leukotriene A4 Hydrolase/LTA4H protein, expressed by HEK293 , with C-6*His labeled tag.

The jADRB1 protein, a member of the beta-adrenergic receptor family, plays a critical role in mediating the activation of adenylate cyclase by catecholamines through G protein signaling. It exhibits similar affinity for binding to epinephrine and norepinephrine. Moreover, ADRB1 protein facilitates Ras activation via G(s)-alpha- and cAMP-mediated signaling pathways. Interestingly, it is also involved in regulating sleep/wake behaviors. ADRB1 protein directly interacts with RAPGEF2 through its C-terminus PDZ motif and further interacts with GOPC, MAGI3, and DLG4. Moving on to the Leukotriene A4 Hydrolase/LTA4H Protein, it is a bifunctional zinc metalloenzyme that possesses both epoxide hydrolase (EH) and aminopeptidase activities. LTA4H acts as an epoxide hydrolase, catalyzing the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4). Additionally, it demonstrates aminopeptidase activity, specifically targeting N-terminal arginines of various synthetic tripeptides. Apart from its pro-inflammatory EH activity, LTA4H may counteract inflammation through its aminopeptidase activity by inactivating the neutrophil attractant Pro-Gly-Pro (PGP), a bioactive collagen fragment generated by matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Furthermore, LTA4H is involved in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, which are lipid mediators with potent anti-inflammatory and pro-resolving actions.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P09960-1 (M1-D611)

4048  [NCBI]

MPEIVDTCSLASPASVCRTKHLHLRCSVDFTRRTLTGTAALTVQSQEDNLRSLVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSAYEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVTAMLVGKDLKVD

60-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM MES, 2 mM EDTA, 20% Glycerol, pH 5.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.