2. Recombinant Proteins
  3. Receptor Proteins Biotinylated Proteins
  4. Leukocyte Immunoglobin-like Receptors
  5. Leukocyte Ig-Like Receptor B4
  6. LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, Domain 1, HEK293, His-Avi)

LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, Domain 1, HEK293, His-Avi)

Cat. No.: HY-P700779
Handling Instructions

The LILRB4/CD85k/ILT3 protein is a multifaceted inhibitory receptor in immune regulation that significantly downregulates multiple immune responses. As a receptor for FN1 and integrin ITGAV/ITGB3, LILRB4/ILT3 inhibits IgE-mediated mast cell activation, KITLG/SCF-mediated mast cell activation, and antibody production by memory/marginal zone B cells through the ITGAV/ITGB3 interaction. LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, Domain 1, HEK293, His-Avi) is the recombinant human-derived LILRB4/CD85k/ILT3 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, Domain 1, HEK293, His-Avi) is 97 a.a., with molecular weight of 14.5-20 kDa.

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Description

The LILRB4/CD85k/ILT3 protein is a multifaceted inhibitory receptor in immune regulation that significantly downregulates multiple immune responses. As a receptor for FN1 and integrin ITGAV/ITGB3, LILRB4/ILT3 inhibits IgE-mediated mast cell activation, KITLG/SCF-mediated mast cell activation, and antibody production by memory/marginal zone B cells through the ITGAV/ITGB3 interaction. LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, Domain 1, HEK293, His-Avi) is the recombinant human-derived LILRB4/CD85k/ILT3 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, Domain 1, HEK293, His-Avi) is 97 a.a., with molecular weight of 14.5-20 kDa.
Background

LILRB4/CD85k/ILT3, an inhibitory receptor intricately involved in immune regulation, plays a crucial role in down-regulating immune responses. It serves as a receptor for FN1 and integrin ITGAV/ITGB3, exerting inhibitory effects on IgE-mediated mast cell activation and KITLG/SCF-mediated mast cell activation. Through its interaction with ITGAV/ITGB3, LILRB4/ILT3 further inhibits antibody production by memory and marginal zone B cells, likely by suppressing their differentiation into plasma cells. This multifaceted receptor extends its inhibitory influence to diverse immune functions, such as suppressing IFNG production by CD8 T cells, CD4 T cells, and natural killer cells, as well as inhibiting antigen presentation by dendritic cells to T cells, preventing T cell activation. Additionally, LILRB4/ILT3 effectively inhibits lipopolysaccharide-mediated neutrophil-dependent vascular injury and contributes to the suppression of the allergic inflammatory response by impeding the infiltration of neutrophils and eosinophils while preventing mast cell degranulation. Its interactions, particularly when tyrosine phosphorylated, with SH2 domain-containing phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 enhance the inhibition of mast cell activation.
Species

Human
Source

HEK293
Tag

C-Avi;C-His
Accession

AAH26309.1 (Q22-T118)
Gene ID
Molecular Construction
N-term
LILRB4 (Q22-T118)
Accession # AAH26309.1
His-Avi
C-term
Synonyms
HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Molecular Weight

14.5-20 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

LILRB4/CD85k/ILT3 Protein, Human (Biotinylated, Domain 1, HEK293, His-Avi) Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
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