LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi)

LRRC15 protein actively modulates SARS-CoV-2 infectivity by directly interacting with the spike protein, inhibiting ACE2(+) cell infection. While not an entry receptor, LRRC15, expressed on nearby cells, sequesters virions, and its direct interaction with the spike protein's RBD domain leads to virion sequestration at the cell surface. LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRRC15 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

The LRRC15 protein modulates the infectivity of SARS-CoV-2 by interacting with its spike protein. It does not function as an entry receptor for SARS-CoV-2, but instead, when expressed on nearby cells, it sequesters virions and inhibits SARS-CoV-2 infection of ACE2(+) cells in a trans manner. Furthermore, LRRC15 protein directly interacts with the RBD domain of the human coronavirus SARS-CoV-2 spike protein, leading to virion sequestration at the cell surface.

Immobilized Anti-LRRC15 Antibody, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC₅₀ of ≤68.5ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q8TF66-1 (Y22-G538)

131578  [NCBI]

YHGCPSECTCSRASQVECTGARIVAVPTPLPWNAMSLQILNTHITELNESPFLNISALIALRIEKNELSRITPGAFRNLGSLRYLSLANNKLQVLPIGLFQGLDSLESLLLSSNQLLQIQPAHFSQCSNLKELQLHGNHLEYIPDGAFDHLVGLTKLNLGKNSLTHISPRVFQHLGNLQVLRLYENRLTDIPMGTFDGLVNLQELALQQNQIGLLSPGLFHNNHNLQRLYLSNNHISQLPPSVFMQLPQLNRLTLFGNSLKELSPGIFGPMPNLRELWLYDNHISSLPDNVFSNLRQLQVLILSRNQISFISPGAFNGLTELRELSLHTNALQDLDGNVFRMLANLQNISLQNNRLRQLPGNIFANVNGLMAIQLQNNQLENLPLGIFDHLGKLCELRLYDNPWRCDSDILPLRNWLLLNQPRLGTDTVPVCFSPANVRGQSLIIINVNVAVPSVHVPEVPSYPETPWYPDTPSYPDTTSVSSTTELTSPVEDYTDLTTIQVTDDRSVWGMTQAQSG

70-80 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.