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  3. LSM4 Protein, Human (His)

The LSM4 protein plays a crucial role in pre-mRNA splicing, participating in the U4/U6-U5 tri-snRNP complex during spliceosome assembly, and as a component of the precatalytic spliceosome (spliceosome B complex).In the heptameric LSM2-8 complex, LSM4 specifically binds to the 3' U region of U6 snRNA.LSM4 Protein, Human (His) is the recombinant human-derived LSM4 protein, expressed by E.coli , with N-6*His labeled tag.

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Description

The LSM4 protein plays a crucial role in pre-mRNA splicing, participating in the U4/U6-U5 tri-snRNP complex during spliceosome assembly, and as a component of the precatalytic spliceosome (spliceosome B complex).In the heptameric LSM2-8 complex, LSM4 specifically binds to the 3' U region of U6 snRNA.LSM4 Protein, Human (His) is the recombinant human-derived LSM4 protein, expressed by E.coli , with N-6*His labeled tag.

Background

LSM4 protein plays a crucial role in pre-mRNA splicing, functioning as a key component of the U4/U6-U5 tri-snRNP complex involved in spliceosome assembly and the precatalytic spliceosome (spliceosome B complex). Alongside LSM2, LSM3, LSM5, LSM6, LSM7, and LSM8, LSM4 forms the heptameric LSM2-8 complex, which specifically binds to the 3'-terminal U-tract of U6 snRNA. This complex is an integral part of the U4/U6-U5 tri-snRNP complex, a fundamental building block in the intricate machinery orchestrating spliceosome assembly. The U4/U6-U5 tri-snRNP complex comprises various snRNAs and associated proteins, underscoring LSM4's essential contribution to the complex and highly regulated process of pre-mRNA splicing.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q9Y4Z0 (M1-Q139)

Gene ID
Molecular Construction
N-term
6*His
LSM4 (M1-Q139)
Accession # Q9Y4Z0
C-term
Synonyms
U6 snRNA-Associated Sm-Like Protein LSm4; Glycine-Rich Protein; GRP; LSM4
AA Sequence

MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKGRGRGGLQQQKQQKGRGMGGAGRGVFGGRGRGGIPGTGRGQPEKKPGRQAGKQ

Molecular Weight

Approximately 17.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

LSM4 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
LSM4 Protein, Human (His)
Cat. No.:
HY-P70944
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