MCP-5/CCL12 Protein, Mouse (CHO)

MCP-5/CCL12 protein, Mouse (CHO) is a potent monocyte-active chemokine that binds to the CCR2 chemokine receptor and is involved in allergic inflammation and host responses to pathogens. MCP-5/CCL12 protein, Mouse (CHO) is a recombinant mouse MCP-5/CCL12 (G23-G104) expressed by CHO^[1].

CCL12, also known as monocyte chemotactic protein 5 (MCP-5), belongs to the CC chemokine cluster on mouse chromosome 11. CCL12 is a potent peripheral blood mononuclear cell chemokine that attracts eosinophils, monocytes and lymphocytes but not neutrophils. It induces calcium flux in peripheral blood mononuclear cells, but not in purified mouse eosinophils or neutrophils. CCL12 is a potent monocyte-active chemokine that signals through CCR2. Involved in allergic inflammation and host response to pathogens, and may play a key role in the early stages of allergic pulmonary inflammation^[1][2].

CCL12 (50 ng/mL) can induce fibroblast migration in wild-type Balb/c mice. However, fibroblasts from CCR2^-/- mice are unable to migrate to CCL12. CCL12 expression increases at day 1 after fluorescein isothiocyanate (FITC)-induced fibrosis, peaks at day 3 after FITC, and remains elevated until day 21. And neutralization of CCL12 in wild-type mice significantly prevents FITC-induced fibrosis^[3].

The ED₅₀ is <0.5 μg/mL as measured by CHO-K1/Gα15/mCCR2 cells (human Gα15 and mouse CCR2 stably expressed in CHO-K1 cells).

Mouse

CHO

Tag Free

Q62401 (G23-G104)

20293  [NCBI]

GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

9.3-11 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sarafi MN,et al. Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1. J Exp Med. 1997 Jan 6;185(1):99-109.  [Content Brief]

  [2]. She S, et al. Functional Roles of Chemokine Receptor CCR2 and Its Ligands in Liver Disease. Front Immunol. 2022 Feb 25;13:812431.

  [3]. Bethany B Moore, et al. The role of CCL12 in the recruitment of fibrocytes and lung fibrosis. Am J Respir Cell Mol Biol. 2006 Aug;35(2):175-81.  [Content Brief]