MCP-5/CCL12 Protein, Mouse (P. pastoris, His)

MCP-5/CCL12 protein promotes CCR2 chemokine receptor binding and contributes to physiological processes such as angiogenesis, inflammation, and monocyte chemotaxis.It is located extracellularly and is widely expressed in tissues such as the central nervous system.MCP-5/CCL12 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived MCP-5/CCL12 protein, expressed by P.pastoris , with C-His labeled tag.

MCP-5/CCL12 protein serves a pivotal role by enabling CCR2 chemokine receptor binding activity, thus contributing to various physiological processes. It plays a significant part in angiogenesis, inflammatory responses, and the chemotaxis of monocytes. Predicted to be located in the extracellular region and active in the extracellular space, this protein exhibits a broad expression pattern in tissues such as the central nervous system and retina. The human ortholog(s) of this gene are implicated in several diseases, including Henoch-Schoenlein purpura, aggressive periodontitis, autoimmune diseases, gastrointestinal system cancers, and tuberculosis, underlining its potential involvement in diverse pathological conditions. The orthologous relationship with human CCL2 and CCL8 emphasizes the evolutionary conservation and functional relevance of this chemokine protein across species.

Determined by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A .The ED₅₀ for this effect is 14.24 ng/mL, corresponding to a specific activity is 7.02×10⁴ U/mg.

Mouse

P. pastoris

C-His

NP_035461.2 (G23-G104)

20293  [NCBI]

GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG

Approximately 10.7 kDa.

Greater than 90 % as determined by SDS-PAGE.

Lyophilized powder.

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.