METAP2/Methionine aminopeptidase 2 Protein, Human (Sf9, His)

METAP2/Methionine aminopeptidase 2 protein removes N-terminal methionine from nascent proteins. It preferentially cleaves when the second residue is small and uncharged. METAP2 has higher catalytic activity for Met-Val peptides than METAP1, suggesting its role in processing Met-Val and Met-Thr sequences in vivo. It also protects EIF2S1 from phosphorylation by inhibitory kinases, regulating protein synthesis. METAP2/Methionine aminopeptidase 2 Protein, Human (Sf9, His) is the recombinant human-derived METAP2/Methionine aminopeptidase 2 protein, expressed by Sf9 insect cells , with N-6*His labeled tag.

METAP2/Methionine aminopeptidase 2 protein functions by cotranslationally removing the N-terminal methionine from nascent proteins. This N-terminal methionine cleavage occurs when the second residue in the primary sequence is small and uncharged, such as Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val. The catalytic activity of human METAP2 towards Met-Val peptides is significantly higher than that of METAP1, suggesting its responsibility for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. In addition, METAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases like EIF2AK2/PKR and EIF2AK1/HCR, thus playing a critical role in regulating protein synthesis.

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC. The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 that incubate at room temperature in kinetic mode for 5 minutes . The specific activity is 120.155 pmol/min/µg.

Human

Sf9 insect cells

N-6*His

P50579-1 (A2-Y478)

10988  [NCBI]

AGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY

Approximately 72.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.