MMP-12 Protein, Human

MMP-12 protein is potentially engaged in tissue injury and remodeling, displaying significant elastolytic activity. It shows flexibility at the P1' site, accommodating both large and small amino acids, yet favoring leucine. At the P1 site, it prefers aromatic or hydrophobic residues, with a tendency for small hydrophobic residues like alanine in the P3 position. MMP-12 Protein, Human is the recombinant human-derived MMP-12 protein, expressed by E. coli , with tag free. The total length of MMP-12 Protein, Human is 163 a.a., with molecular weight of ~18 kDa.

The MMP-12 protein is potentially involved in tissue injury and remodeling and possesses notable elastolytic activity. It demonstrates the ability to accept both large and small amino acids at the P1' site, although it exhibits a preference for leucine. At the P1 site, it favors aromatic or hydrophobic residues, with a preference for small hydrophobic residues like alanine in the P3 position.

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 and the specific activity is > 800 pmoles/min/µg.

Human

E. coli

Tag Free

P39900 (G106-N268)

4321  [NCBI]

Approximately 18 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 2 mM CaCl₂, 250 mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.