NA/Neuraminidase Protein, H5N1 (ABU94738, sf9, His)

Neuraminidase (NA) is described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. Whereby, NA facilitates viral invasion of the upper airways, facilitates virus release during virus budding, prevents self-aggregation and ensures the efficient spread of the progeny virus from cell to cell, and also plays a role in the determination of host range restriction on replication and virulence. NA/Neuraminidase Protein, H5N1 (ABU94738, sf9, His) is the recombinant Virus-derived NA/Neuraminidase protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of NA/Neuraminidase Protein, H5N1 (ABU94738, sf9, His) is 414 a.a., with molecular weight of ~51.1 kDa.

Neuraminidase (NA) is described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. NA may facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. NA catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates, cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release and additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell or the infection would be limited to one round of replication. NA also plays a role in the determination of host range restriction on replication and virulence^[1][2].

Virus

Sf9 insect cells

C-His

ABU94738 (H36-K449)

/

Approximately 51.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.