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NARS Protein, Human (sf9, His)

Cat. No.: HY-P74738
Handling Instructions

NARS proteins catalyze a two-step process that activates asparagine with ATP to form Asn-AMP and transfers it to the acceptor terminus of tRNA (Asn). NARS Protein, Human (sf9, His) is the recombinant human-derived NARS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of NARS Protein, Human (sf9, His) is 548 a.a., with molecular weight of ~65.3 kDa.

For research use only. We do not sell to patients.

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Description

NARS proteins catalyze a two-step process that activates asparagine with ATP to form Asn-AMP and transfers it to the acceptor terminus of tRNA (Asn). NARS Protein, Human (sf9, His) is the recombinant human-derived NARS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of NARS Protein, Human (sf9, His) is 548 a.a., with molecular weight of ~65.3 kDa.

Background

NARS, a pivotal enzyme in protein synthesis, plays a crucial role in the attachment of asparagine to tRNA(Asn) through a meticulously orchestrated two-step process. Initially, asparagine undergoes activation by ATP, resulting in the formation of Asn-AMP. Subsequently, this activated asparagine is diligently transferred to the acceptor end of tRNA(Asn). Beyond its fundamental involvement in protein synthesis, NARS assumes an additional role as a signaling molecule capable of inducing the migration of CCR3-expressing cells. The multifaceted functions of NARS extend to the realm of developmental processes, where it emerges as an indispensable contributor to the proper proliferation of radial glial cells, particularly in the development of the cerebral cortex. The intricate interplay of NARS in both protein synthesis and cellular signaling underscores its significance in fundamental biological processes.

Species

Human

Source

Sf9 insect cells

Tag

N-His

Accession

O43776 (M1-P548)

Gene ID
Molecular Construction
N-term
His
NARS (M1-P548)
Accession # O43776
C-term
Synonyms
Asparagine--tRNA ligase, cytoplasmic; AsnRS; NARS1; NARS; NRS
Molecular Weight

Approximately 65.3 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

NARS Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NARS Protein, Human (sf9, His)
Cat. No.:
HY-P74738
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