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  5. NCAM-1/CD56 Protein, Rat (HEK293, His)

NCAM-1/CD56 Protein, Rat (HEK293, His)

Cat. No.: HY-P73749
COA Handling Instructions

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth.Its interaction with MDK contributes to complex cellular interactions in neural development.NCAM-1/CD56 Protein, Rat (HEK293, His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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5 μg $45 In-stock
10 μg $70 In-stock
50 μg $180 In-stock
100 μg $305 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The NCAM-1/CD56 protein is a key cell adhesion molecule that plays a key role in neuron-neuron adhesion, neurite fasciculation, and neurite outgrowth.Its interaction with MDK contributes to complex cellular interactions in neural development.NCAM-1/CD56 Protein, Rat (HEK293, His) is the recombinant rat-derived NCAM-1/CD56 protein, expressed by HEK293 , with C-His labeled tag.

Background

NCAM-1/CD56 Protein, a cell adhesion molecule, plays a pivotal role in various neuronal processes, including neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurites. It interacts with MDK, contributing to the intricate cellular interactions in neural development. Furthermore, NCAM-1 is part of a complex involving SLC39A6, SLC39A10, and NCAM1 itself, which regulates NCAM1 phosphorylation and facilitates its integration into focal adhesion complexes during epithelial-to-mesenchymal transition. This underscores the dynamic involvement of NCAM-1 in cell adhesion and signaling events, emphasizing its importance in both neural development and cellular processes associated with epithelial-to-mesenchymal transition.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED50 this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×103 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a human T-lymphocyte leukemia cells. The ED50 this effect is 0.839 μg/mL, corresponding to a specific activity is 1.19×103 units/mg.
Species

Rat

Source

HEK293

Tag

C-His

Accession

P13596 (L20-T721)

Gene ID
Molecular Construction
N-term
NCAM-1 (L20-T721)
Accession # P13596
His
C-term
Synonyms
CD56; MSK39; NCAM-1; Neural cell adhesion molecule 1; NCAM
AA Sequence

LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

Molecular Weight

115-130 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 0.15M NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NCAM-1/CD56 Protein, Rat (HEK293, His)
Cat. No.:
HY-P73749
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