NEGR1 Protein, Human (HEK293, His)

The NEGR1 protein is associated with cell adhesion, suggesting a role in important cellular interactions. Its potential function as a transneural growth-promoting factor in regenerating axonal sprouting suggests a dynamic role in promoting neuronal growth. NEGR1 Protein, Human (HEK293, His) is the recombinant human-derived NEGR1 protein, expressed by HEK293 , with C-His labeled tag. The total length of NEGR1 Protein, Human (HEK293, His) is 287 a.a., with molecular weight of 45-48 KDa.

NEGR1 Protein appears to be implicated in cell adhesion, suggesting a potential role in mediating interactions critical for cellular processes. Its putative function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain indicates a dynamic role in facilitating neuronal growth. The proposed similarity to other growth-promoting factors implies NEGR1's potential involvement in regenerative processes, particularly in the context of axon sprouting in the brain. Elucidating the specific mechanisms through which NEGR1 participates in cell adhesion and contributes to regenerative axon sprouting could offer valuable insights into its role in neural development and regeneration. Further exploration of NEGR1's functions may provide a better understanding of its potential implications in neuronal plasticity and regenerative processes within the mammalian brain.

Measured by its ability to the proliferation of SH-SY5Y Human neuroblastoma cells. The ED₅₀ for this effect is 14.38μg/mL, corresponding to a specific activity is 69.541 Unit/mg.

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  Measured by its ability to the proliferation of SH-SY5Y Human neuroblastoma cells.The ED₅₀ for this effect is 14.38μg/mL, corresponding to a specific activity is 69.541 Unit/mg.

Human

HEK293

C-His

Q7Z3B1-1 (V38-G324)

257194  [NCBI]

VDFPWAAVDNMMVRKGDTAVLRCYLEDGASKGAWLNRSSIIFAGGDKWSVDPRVSISTLNKRDYSLQIQNVDVTDDGPYTCSVQTQHTPRTMQVHLTVQVPPKIYDISNDMTVNEGTNVTLTCLATGKPEPSISWRHISPSAKPFENGQYLDIYGITRDQAGEYECSAENDVSFPDVRKVKVVVNFAPTIQEIKSGTVTPGRSGLIRCEGAGVPPPAFEWYKGEKKLFNGQQGIIIQNFSTRSILTVTNVTQEHFGNYTCVAANKLGTTNASLPLNPPSTAQYGITG

Approximately 42-55 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.