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Neurolysin Protein, Human (His)

Cat. No.: HY-P75938
COA Handling Instructions

Neurolysin protein is a multifunctional enzyme that hydrolyzes oligopeptides such as neurotensin, bradykinin, and dynorphin A. In addition to its role in oligopeptide metabolism, Neurolysin also modulates cannabinoid signaling pathways by promoting the degradation of vasopressin, an antagonist peptide of the cannabinoid receptor CNR1. Neurolysin Protein, Human (His) is the recombinant human-derived Neurolysin protein, expressed by E. coli , with N-His labeled tag. The total length of Neurolysin Protein, Human (His) is 667 a.a., with molecular weight of ~67 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Neurolysin protein is a multifunctional enzyme that hydrolyzes oligopeptides such as neurotensin, bradykinin, and dynorphin A. In addition to its role in oligopeptide metabolism, Neurolysin also modulates cannabinoid signaling pathways by promoting the degradation of vasopressin, an antagonist peptide of the cannabinoid receptor CNR1. Neurolysin Protein, Human (His) is the recombinant human-derived Neurolysin protein, expressed by E. coli , with N-His labeled tag. The total length of Neurolysin Protein, Human (His) is 667 a.a., with molecular weight of ~67 kDa.

Background

Neurolysin protein serves as a versatile enzyme with the ability to hydrolyze various oligopeptides, including neurotensin, bradykinin, and dynorphin A. Beyond its role in oligopeptide metabolism, Neurolysin assumes a regulatory function in the cannabinoid signaling pathway. It accomplishes this by facilitating the degradation of hemopressin, an antagonist peptide that interacts with the cannabinoid receptor CNR1. This dual role positions Neurolysin as a key player in both the enzymatic breakdown of biologically active peptides and the modulation of cannabinoid receptor signaling through the regulation of hemopressin levels.

Biological Activity

Measured by its ability to cleave a fluorogenic peptide substrate (7-methoxycoumarin-4-yl) acetyl Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH and the specific activity is > 100 pmol/min/μg.

Species

Human

Source

E. coli

Tag

N-His

Accession

Q9BYT8 (S38-P704)

Gene ID
Molecular Construction
N-term
His
Neurolysin (S38-P704)
Accession # Q9BYT8
C-term
Synonyms
Neurolysin, mitochondrial; Microsomal endopeptidase; MEP; NLN; AGTBP; KIAA1226
AA Sequence

SSYTVAGRNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVERTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKPEARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKAELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEENTIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVASRLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGHLAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDMLHNFLKREPNQKAFLMSRGLHAP

Molecular Weight

Approximately 67 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Neurolysin Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Neurolysin Protein, Human (His)
Cat. No.:
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