Neuropilin-1 Protein, Mouse (HEK293, Fc)

Neuropilin-1 protein is an important receptor critical for cardiovascular system development, angiogenesis, neuronal circuit formation, and non-nervous system organogenesis.It mediates the chemical pulse activity of signaling proteins, recognizes C-terminal regular motifs on ligands, and promotes cellular internalization and vascular leakage.Neuropilin-1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Neuropilin-1 protein, expressed by HEK293 , with C-mFc labeled tag.

Neuropilin-1 Protein serves as a receptor crucial for the development of the cardiovascular system, angiogenesis, the formation of specific neuronal circuits, and organogenesis outside the nervous system. Functioning as a mediator of semaphorins' chemorepulsant activity, it recognizes the C-end rule (CendR) motif R/KXXR/K on its ligands, facilitating cellular internalization and vascular leakage. Neuropilin-1 binds to semaphorin 3A (SEMA3A), the PLGF-2 isoform of PGF, and the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR enhances VEGF165 binding to KDR and increases chemotaxis. The protein regulates VEGF-induced angiogenesis and initiates a signaling pathway crucial for motor neuron axon guidance and cell body migration during embryonic development. Additionally, Neuropilin-1 plays a role in regulating mitochondrial iron transport through interaction with ABCB8/MITOSUR. It forms homodimers and heterodimers with NRP2, binds PLXNB1, and interacts with FER, VEGFA, and ABCB8/MITOSUR in mitochondria.

Immobilized Mouse VEGF164 at 2 μg/ml (100 μl/well) can bind Mouse Neuropilin-1 mFc, the EC₅₀ of Mouse Neuropilin-1 mFc is 4.0-20.0 ng/mL.

Mouse

HEK293

C-mFc

P97333 (M1-P856)

18186  [NCBI]

Approximately 120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.