1. Recombinant Proteins
  2. Viral Proteins
  3. Dengue Virus Proteins
  4. DENV Non-structural Protein 1 (NS1)
  5. NS1 Protein, Dengue virus 2 (HEK293, His)

NS1 Protein, Dengue virus 2 (HEK293, His)

Cat. No.: HY-P73740
SDS COA Handling Instructions

NS5 Protein is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). NS5 plays a fundamental role in flavivirus replication, viral RNA methylation, RNA polymerization, and host immune system evasion. NS1 Protein, Dengue virus 2 (HEK293, His) is the recombinant Virus-derived NS1 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $45 In-stock
10 μg $75 In-stock
50 μg $200 In-stock
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Description

NS5 Protein is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). NS5 plays a fundamental role in flavivirus replication, viral RNA methylation, RNA polymerization, and host immune system evasion. NS1 Protein, Dengue virus 2 (HEK293, His) is the recombinant Virus-derived NS1 protein, expressed by HEK293 , with N-His labeled tag.

Background

Non-structural protein 5 (NS5) is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). The DENV-NS5 RdRp domain contributes to the viral replication stages, whereas the MTase initiates viral RNA capping and facilitates polyprotein translation. NS5 plays a fundamental role in viral RNA methylation, RNA polymerization, and host immune system evasion. It functions as a scaffold protein with different binding sites for the host STAT2 and ERC1 proteins, but sharing a requirement for UBR4. NS5 also interacts with hostcell proteins as hSTAT2 and contributes to the evasion of the type I interferon (IFN)-mediated innate immune response, which is the first step of host-cell defense against viral infections. NS5 is a potent antagonist of type I IFN signaling of the DENV proteins by suppressing the human JAK-STAT signaling[1][2][3].

Species

Virus

Source

HEK293

Tag

N-His

Accession

AAC59275.1 (D776-A1127)

Gene ID

/

Molecular Construction
N-term
His
NS1 (D776-A1127)
Accession # AAC59275.1
C-term
Synonyms
DEN-2; Dengue NS1 protein; Dengue virus Type 2; DENV2; DENV2-NS1
Molecular Weight

Approximately 42.3 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

NS1 Protein, Dengue virus 2 (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NS1 Protein, Dengue virus 2 (HEK293, His)
Cat. No.:
HY-P73740
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