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  3. Orm2 Protein, Human (HEK293, His)

Orm2 Protein, Human (HEK293, His) produced in HEK293 cells, is a recombinant human Alpha-1-acid glycoprotein 2 with His tag. Alpha-1-acid glycoprotein 2 belongs to the immunocalin family, which is a lipocalin subfamily with immunomodulatory functions.

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Description

Orm2 Protein, Human (HEK293, His) produced in HEK293 cells, is a recombinant human Alpha-1-acid glycoprotein 2 with His tag. Alpha-1-acid glycoprotein 2 belongs to the immunocalin family, which is a lipocalin subfamily with immunomodulatory functions[1].

Background

Alpha-1-acid glycoprotein 2 (Orosomucoid, ORM) is an acute-phase protein that belongs to the immunocalin subfamily, a group of small-molecule-binding proteins with immunomodulatory functions. Alpha-1-acid glycoprotein 2 exerts anti-inflammatory effects by modulating microglial activation and migration during brain inflammation[1].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

P19652 (Q19-S201)

Gene ID
Molecular Construction
N-term
Orm2 (Q19-S201)
Accession # P19652
6*His
C-term
Synonyms
rHualpha-1-acid glycoprotein 2, His; AGP2; ORM2; alpha-1-acid glycoprotein 2
AA Sequence

QIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQNQCFYNSSYLNVQRENGTVSRYEGGREHVAHLLFLRDTKTLMFGSYLDDEKNWGLSFYADKPETTKEQLGEFYEALDCLCIPRSDVMYTDWKKDKCEPLEKQHEKERKQEEGESHHHHHH

Molecular Weight

25-50 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Orm2 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Orm2 Protein, Human (HEK293, His)
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