Osteopontin/OPN Protein, Mouse (HEK293, His)

Osteopontin/OPN Protein, a crucial non-collagenous bone protein, binds strongly to hydroxyapatite, integral to the mineralized matrix.It likely plays a vital role in mediating cell-extracellular matrix interactions.Moreover, as a cytokine, OPN efficiently induces interferon-gamma and interleukin-12 production while inhibiting interleukin-10, pivotal in promoting type I immunity.Osteopontin/OPN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Osteopontin/OPN protein, expressed by HEK293 , with C-His labeled tag.

Osteopontin (OPN) protein, a vital non-collagenous bone protein, demonstrates a strong affinity for hydroxyapatite and serves as an integral component of the mineralized matrix. It likely plays a crucial role in facilitating interactions between cells and the extracellular matrix. Additionally, OPN acts as a cytokine, effectively stimulating the production of interferon-gamma and interleukin-12, while simultaneously suppressing the production of interleukin-10. These functions are essential in the pathway that promotes type I immunity.

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Human Osteopontin coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.08978 μg/mL, corresponding to a specific activity is 1.11×10⁴ units/mg.

Mouse

HEK293

C-His

P10923 (L17-N294)

20750  [NCBI]

50-60 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.