PACSIN1 Protein, Human (HEK293, His)

The PACSIN1 protein has multiple functions in cell dynamics, reorganizing microtubules and the actin cytoskeleton. PACSIN1 interacts with MAPT, reduces microtubule stability and inhibits MAPT-induced polymerization. PACSIN1 Protein, Human (HEK293, His) is the recombinant human-derived PACSIN1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PACSIN1 Protein, Human (HEK293, His) is 444 a.a., with molecular weight of ~52.0 kDa.

PACSIN1 Protein plays a multifaceted role in cellular dynamics, contributing significantly to the reorganization of both the microtubule and actin cytoskeletons. Through its interaction with MAPT, PACSIN1 orchestrates the reorganization of the microtubule cytoskeleton, leading to a reduction in microtubule stability and the inhibition of MAPT-induced microtubule polymerization. Simultaneously, PACSIN1 participates in cellular transport processes by recruiting DNM1, DNM2, and DNM3 to membranes. In the realm of neuron morphogenesis, PACSIN1 interacts with COBL and WASL, recruiting COBL to the cell cortex and influencing the reorganization of the actin cytoskeleton. This intricate network extends to the regulation of neurite formation, branching, and length, crucial for normal synaptic vesicle endocytosis and neurotransmission. Anchoring to membranes via its F-BAR domain, PACSIN1 mediates membrane tubulation and exhibits versatility in forming heterooligomers with other PACSINs, further highlighting its central role in diverse cellular processes. Interactions with MAPT, TRPV4, SYNJ1, WASL, DNM2, DNM3, COBL, DBNL, EHD1, and EHD3 underscore the complexity of PACSIN1's molecular engagements, contributing to its multifunctional significance in cellular physiology.

Human

HEK293

C-6*His

Q9BY11 (M1-I444)

29993  [NCBI]

MSSSYDEASLAPEETTDSFWEVGNYKRTVKRIDDGHRLCNDLMNCVQERAKIEKAYGQQLTDWAKRWRQLIEKGPQYGSLERAWGAIMTEADKVSELHQEVKNNLLNEDLEKVKNWQKDAYHKQIMGGFKETKEAEDGFRKAQKPWAKKMKELEAAKKAYHLACKEEKLAMTREMNSKTEQSVTPEQQKKLQDKVDKCKQDVQKTQEKYEKVLEDVGKTTPQYMENMEQVFEQCQQFEEKRLVFLKEVLLDIKRHLNLAENSSYIHVYRELEQAIRGADAQEDLRWFRSTSGPGMPMNWPQFEEWNPDLPHTTTKKEKQPKKAEGVALTNATGAVESTSQAGDRGSVSSYDRGQPYATEWSDDESGNPFGGSETNGGANPFEDDSKGVRVRALYDYDGQEQDELSFKAGDELTKLGEEDEQGWCRGRLDSGQLGLYPANYVEAI

Approximately 52.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.