PAH Protein, Human (D415N, sf9, His)

PAH protein is a key enzyme involved in amino acid metabolism. Its main function is to catalyze the hydroxylation of L-phenylalanine and convert it into L-tyrosine. PAH Protein, Human (D415N, sf9, His) is the recombinant human-derived PAH protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PAH Protein, Human (D415N, sf9, His) is 452 a.a., with molecular weight of ~52 kDa.

PAH protein is a key enzyme involved in amino acid metabolism. Its primary function is to catalyze the hydroxylation of L-phenylalanine, converting it into L-tyrosine. This enzymatic activity is a critical step in the conversion of one amino acid to another, specifically contributing to the synthesis of tyrosine from phenylalanine. This process is essential for various biological processes that require the production of tyrosine, which serves as a building block for the synthesis of important molecules like neurotransmitters, thyroid hormones, and melanin. The activity of PAH protein plays a pivotal role in maintaining proper amino acid balance and supporting various physiological functions in the body.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P00439 (M1-K452,D415N)

5053  [NCBI]

MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYNPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIK

Approximately 52 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.