PARK7/DJ-1 Protein, Human (GST)

The multifunctional PARK7/DJ-1 protein plays a key role in cellular defense against oxidative stress and cell death. It acts as an oxidative stress sensor, redox-sensitive chaperone and protease, and participates in neuroprotective mechanisms by stabilizing NFE2L2 and PINK1 proteins. PARK7/DJ-1 Protein, Human (GST) is the recombinant human-derived PARK7/DJ-1 protein, expressed by E. coli , with N-GST labeled tag. The total length of PARK7/DJ-1 Protein, Human (GST) is 188 a.a., with molecular weight of ~46.8 kDa.

PARK7/DJ-1, a multifunctional protein, assumes a pivotal role in cellular defense against oxidative stress and cell death, functioning as an oxidative stress sensor, redox-sensitive chaperone, and protease. It engages in neuroprotective mechanisms, stabilizing NFE2L2 and PINK1 proteins, positively regulating the androgen signaling pathway for male fertility, and modulating cell growth and transformation through its impact on the NF-kappa-B signaling pathway. Despite its disputed role as a protein and nucleotide deglycase, implicated in repairing glycated proteins and nucleotides, PARK7/DJ-1's involvement in a nucleotide repair system named guanine glycation repair (GG repair) is recognized, mitigating damage caused by methylglyoxal and glyoxal. Acting on early glycation intermediates, it prevents the formation of advanced glycation endproducts (AGE) that inflict irreversible damage. Beyond its role in glycation repair, PARK7/DJ-1 maintains mitochondrial morphology and function, facilitates autophagy of dysfunctional mitochondria, regulates expression or stability of mitochondrial uncoupling proteins, and orchestrates protective responses in pancreatic islets. Moreover, it exerts control over inflammatory responses in astrocytes, modulates endocytosis, binds to specific mRNAs, and exhibits metal-binding capabilities, enhancing cellular defense mechanisms against metal toxicity.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

Q99497 (1M-188K)

11315  [NCBI]

MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEVAAQVKAPLVLK

Approximately 46.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.