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  3. PBK/TOPK Protein, Human (sf9, His)

PBK/TOPK Protein, Human (sf9, His)

Cat. No.: HY-P77122
COA Handling Instructions

PBK/TOPK Protein, a mitosis-specific kinase, phosphorylates MAP kinase p38, emphasizing its specific role in cell division. Implicated in lymphoid cell activation, PBK/TOPK has broader impacts on immune responses. When phosphorylated, it forms a complex with TP53, destabilizing TP53 and dampening the G2/M checkpoint in doxorubicin-induced DNA damage, highlighting its multifaceted functions in cell cycle control and genotoxic stress responses. PBK/TOPK Protein, Human (sf9, His) is the recombinant human-derived PBK/TOPK protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

PBK/TOPK Protein, a mitosis-specific kinase, phosphorylates MAP kinase p38, emphasizing its specific role in cell division. Implicated in lymphoid cell activation, PBK/TOPK has broader impacts on immune responses. When phosphorylated, it forms a complex with TP53, destabilizing TP53 and dampening the G2/M checkpoint in doxorubicin-induced DNA damage, highlighting its multifaceted functions in cell cycle control and genotoxic stress responses. PBK/TOPK Protein, Human (sf9, His) is the recombinant human-derived PBK/TOPK protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

PBK/TOPK, a mitosis-specific protein kinase, exhibits activity by phosphorylating MAP kinase p38. Its functional involvement appears to be confined to mitotic processes, emphasizing a specific role during cell division. Additionally, PBK/TOPK is implicated in the activation of lymphoid cells, suggesting a broader impact on immune responses. Notably, when phosphorylated, PBK/TOPK forms a complex with TP53, resulting in TP53 destabilization and a dampening of the G2/M checkpoint in response to DNA damage induced by doxorubicin. This intricate regulation underscores the multifaceted functions of PBK/TOPK in cell cycle control and cellular responses to genotoxic stress.

Biological Activity

No Kinase Activity

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

Q96KB5-1 (M1-V322)

Gene ID
Molecular Construction
N-term
PBK (M1-V322)
Accession # Q96KB5-1
His
C-term
Synonyms
Lymphokine-activated killer T-cell-originated protein kinase; CT84; Nori-3; SPK; TOPK
Molecular Weight

35-45 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PBK/TOPK Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PBK/TOPK Protein, Human (sf9, His)
Cat. No.:
HY-P77122
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