PBK/TOPK Protein, Human (sf9, His)

PBK/TOPK Protein, a mitosis-specific kinase, phosphorylates MAP kinase p38, emphasizing its specific role in cell division. Implicated in lymphoid cell activation, PBK/TOPK has broader impacts on immune responses. When phosphorylated, it forms a complex with TP53, destabilizing TP53 and dampening the G2/M checkpoint in doxorubicin-induced DNA damage, highlighting its multifaceted functions in cell cycle control and genotoxic stress responses. PBK/TOPK Protein, Human (sf9, His) is the recombinant human-derived PBK/TOPK protein, expressed by Sf9 insect cells , with C-His labeled tag.

PBK/TOPK, a mitosis-specific protein kinase, exhibits activity by phosphorylating MAP kinase p38. Its functional involvement appears to be confined to mitotic processes, emphasizing a specific role during cell division. Additionally, PBK/TOPK is implicated in the activation of lymphoid cells, suggesting a broader impact on immune responses. Notably, when phosphorylated, PBK/TOPK forms a complex with TP53, resulting in TP53 destabilization and a dampening of the G2/M checkpoint in response to DNA damage induced by doxorubicin. This intricate regulation underscores the multifaceted functions of PBK/TOPK in cell cycle control and cellular responses to genotoxic stress.

No Kinase Activity

Human

Sf9 insect cells

C-His

Q96KB5-1 (M1-V322)

55872  [NCBI]

35-45 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.