PC4/SUB1 Protein, Human (His)

The PC4/SUB1 protein acts as a multifunctional coactivator that cooperates with TAF to promote functional interactions between upstream activators and the general transcription machinery. Its role extends to the potential stability of multiprotein transcription complexes. PC4/SUB1 Protein, Human (His) is the recombinant human-derived PC4/SUB1 protein, expressed by E. coli , with N-His labeled tag. The total length of PC4/SUB1 Protein, Human (His) is 127 a.a., with molecular weight of ~19 kDa.

PC4/SUB1, also known as positive cofactor 4, is a general coactivator with a pivotal role in facilitating functional interactions between upstream activators and the general transcriptional machinery. It functions cooperatively with TAFs (TBP-associated factors) and is implicated in stabilizing multiprotein transcription complexes during gene expression. PC4/SUB1 demonstrates the ability to bind both single-stranded DNA and, in vitro, non-specifically to double-stranded DNA (dsDNA), indicating a versatile DNA-binding capability. Structurally, it forms homodimers, suggesting a cooperative arrangement that may enhance its functional properties. Additionally, PC4/SUB1 interacts with CSTF2, further emphasizing its involvement in diverse cellular processes related to transcriptional regulation. Ongoing research may unveil more insights into the specific mechanisms and regulatory functions of PC4/SUB1 in gene expression.

Human

E. coli

N-His

P53999 (M1-L127)

10923  [NCBI]

MPKSKELVSSSSSGSDSDSEVDKKLKRKKQVAPEKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPEGEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.