PCSK1 Protein, Human (HEK293, His)

The PCSK1 protein is a proprotein convertase that plays a key role in processing hormone and protein precursors at sites with basic amino acid residues. It targets multiple substrates such as POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. PCSK1 Protein, Human (HEK293, His) is the recombinant human-derived PCSK1 protein, expressed by HEK293 , with C-His labeled tag.

The PCSK1 Protein plays a pivotal role in the processing of hormone and other protein precursors, particularly at sites characterized by pairs of basic amino acid residues. Functioning as a proprotein convertase, PCSK1 targets a range of substrates, including POMC, renin, enkephalin, dynorphin, somatostatin, insulin, and AGRP. Through its enzymatic activity, PCSK1 is involved in the proteolytic cleavage and maturation of these precursor molecules, thereby regulating the generation of active hormones and neuropeptides. The diverse substrates highlight the broad impact of PCSK1 on the processing of proteins crucial for various physiological functions, including metabolism, endocrine signaling, and neuropeptide regulation.

Measured by its ability to cleave the fluorogenic peptide 200 µM substrate pERTKR-AMC. that incubate at 37°C temperature for 60 minutes. The specific activity is 116.38 pmol/min/µg.

Human

HEK293

C-10*His

P29120-1/NP_000430.3 (S111-R617)

5122  [NCBI]

SALRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDR

Approximately 60-70 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCL, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.