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  4. PCSK1 Protein, Human (HEK293, His)

PCSK1 Protein, Human (HEK293, His)

Cat. No.: HY-P77462
COA Handling Instructions

The PCSK1 protein is a proprotein convertase that plays a key role in processing hormone and protein precursors at sites with basic amino acid residues. It targets multiple substrates such as POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. PCSK1 Protein, Human (HEK293, His) is the recombinant human-derived PCSK1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The PCSK1 protein is a proprotein convertase that plays a key role in processing hormone and protein precursors at sites with basic amino acid residues. It targets multiple substrates such as POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP. PCSK1 Protein, Human (HEK293, His) is the recombinant human-derived PCSK1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The PCSK1 Protein plays a pivotal role in the processing of hormone and other protein precursors, particularly at sites characterized by pairs of basic amino acid residues. Functioning as a proprotein convertase, PCSK1 targets a range of substrates, including POMC, renin, enkephalin, dynorphin, somatostatin, insulin, and AGRP. Through its enzymatic activity, PCSK1 is involved in the proteolytic cleavage and maturation of these precursor molecules, thereby regulating the generation of active hormones and neuropeptides. The diverse substrates highlight the broad impact of PCSK1 on the processing of proteins crucial for various physiological functions, including metabolism, endocrine signaling, and neuropeptide regulation.

Biological Activity

Measured by its ability to cleave the fluorogenic peptide 200 µM substrate pERTKR-AMC. that incubate at 37°C temperature for 60 minutes. The specific activity is 116.38 pmol/min/µg.

Species

Human

Source

HEK293

Tag

C-10*His

Accession

P29120-1/NP_000430.3 (S111-R617)

Gene ID
Molecular Construction
N-term
PCSK1 (S111-R617)
Accession # P29120-1/NP_000430.3
His
C-term
Synonyms
Neuroendocrine convertase 1; Prohormone convertase 1; PC1; NEC1
AA Sequence

SALRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDR

Molecular Weight

Approximately 60-70 kDa due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCL, 150 mM NaCl, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PCSK1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PCSK1 Protein, Human (HEK293, His)
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