PCSK9 Protein, Rhesus Macaque (HEK293, His)

PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER and LRP8/APOER2 receptors. It promotes LDLR degradation through non-proteolytic mechanisms, disrupts LDLR recycling, induces LDLR ubiquitination, and inhibits APOB degradation. PCSK9 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived PCSK9 protein, expressed by HEK293 , with C-His labeled tag. The total length of PCSK9 Protein, Rhesus Macaque (HEK293, His) is 662 a.a., with molecular weight of ~20 & 62 kDa, respectively.

PCSK9 Protein plays a crucial role in maintaining the balance of cholesterol levels in the blood. It binds to several receptors involved in lipid metabolism, including LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2, and promotes their degradation in acidic compartments inside cells. It enhances the degradation of hepatic LDLR through a non-proteolytic mechanism mediated by clathrin LDLRAP1/ARH pathway. Additionally, it may disrupt the recycling of LDLR, directing it to lysosomes for degradation. PCSK9 can also induce ubiquitination of LDLR, leading to its subsequent breakdown. Furthermore, it inhibits the degradation of APOB, an important component of lipoproteins, through the autophagosome/lysosome pathway, independently of LDLR. In the early secretory pathway, PCSK9 helps dispose of non-acetylated intermediates of BACE1. It also plays a role in regulating the absorption of sodium ions through the ENaC channel by reducing its surface expression through increased proteasomal degradation. Finally, PCSK9 regulates neuronal apoptosis by modulating the levels of LRP8/APOER2 and related anti-apoptotic signaling pathways.

Immobilized Rhesus PCSK9 at 5 µg/mL (100 µL/well) can bind Biotinylated rmLDLR. The ED₅₀ for this effect is 224.2 ng/mL

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  Immobilized Rhesus PCSK9 at 5 µg/mL (100 µL/well) can bind Biotinylated rmLDLR. The ED₅₀ for this effect is 224.2 ng/mL.

Rhesus Macaque

HEK293

C-His

A8T666 (Q31-Q692)

717147  [NCBI]

QEDEDGDYEELVLALRSEEDGLADAPEHGATATFHRCAKDPWRLPGTYVVVLKEETHRSQSERTARRLQAQAARRGYLTKILHVFHHLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPARYRADEYQPPKGGSLVEVYLLDTSIQSDHREIEGRVMVTDFESVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGAGLRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVFNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSRSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHRAGWQLFCRTVWSAHSGPTRMATAVARCAQDEELLSCSSFSRSGKRRGERIEAQGGKRVCRAHNAFGGEGVYAIARCCLLPQVNCSVHTAPPAGASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVIVACEDGWTLTGCSPLPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSKEAVAAVAICCRSRHLVQASQELQ

Approximately 20&62 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.