PD-L1 Protein, Mouse (HEK293, His-Fc)

PD-L1 protein serves as a ligand for the inhibitory receptor PDCD1/PD-1 and is critical for maintaining immune tolerance. This interaction modulates T cell activation, limits effector responses and may stimulate IL10-producing T cell subsets. PD-L1 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived PD-L1 protein, expressed by HEK293 , with C-hFc, C-His labeled tag.

PD-L1 protein plays a crucial role in maintaining immune tolerance to self by acting as a ligand for the inhibitory receptor PDCD1/PD-1. This interaction modulates the activation threshold of T-cells, limiting their effector response and potentially stimulating T-cell subsets that produce interleukin-10 (IL10). However, tumors exploit the PDCD1-mediated inhibitory pathway to attenuate anti-tumor immunity and evade destruction by the immune system, thereby promoting tumor survival. The interaction between PD-L1 and PDCD1/PD-1 inhibits the function of cytotoxic T lymphocytes (CTLs), but blocking this pathway can reverse the exhausted T-cell phenotype and normalize the anti-tumor response, offering a promising strategy for cancer immunotherapy.

1. Measured by its ability to bind mouse PD-1 in functional ELISA.
2. Measured by its binding ability in a functional ELISA. Immobilized Mouse PD-L1 at 25 ng/mL (100μl/well) on the plate can bind Biotinylated Mouse PD-1 (HY-P70640). The ED₅₀ for this effect is 32.43 ng/mL.

Mouse

HEK293

C-hFc;C-His

Q9EP73 (F19-T238)

60533  [NCBI]

Approximately 75-90 kDa band in SDS-PAGE under reducing conditions due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5%mannitol, 0.01% tween-80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.