PDGF-BB Protein, Mouse (P.pastoris, His)

PDGF-BB, a crucial growth factor, regulates diverse cellular processes, including development, proliferation, migration, survival, and chemotaxis. It is vital for tissue proliferation, blood vessel development, and kidney glomeruli formation. PDGF-BB interacts with PDGFRA, PDGFRB, XLKD1, LRP1, and SORL1, highlighting its versatile cellular involvement. PDGF-BB Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived PDGF-BB protein, expressed by P. pastoris , with N-His labeled tag.

PDGF-BB, a crucial growth factor, assumes a central role in regulating diverse cellular processes, encompassing embryonic development, cell proliferation, migration, survival, and chemotaxis. As a potent mitogen for mesenchymal cells, PDGF-BB is indispensable for the normal proliferation and recruitment of pericytes and vascular smooth muscle cells in various tissues, including the central nervous system, skin, lung, heart, and placenta. It is essential for blood vessel development and the formation of kidney glomeruli, highlighting its multifaceted contributions to tissue homeostasis. Furthermore, PDGF-BB plays a pivotal role in wound healing. The intricacies of its signaling are modulated by the formation of antiparallel homodimers, linked by disulfide bonds, and antiparallel heterodimers with PDGFA. These dynamic dimers engage in intricate interactions with PDGFRA and PDGFRB homodimers, as well as heterodimers formed by PDGFRA and PDGFRB. Additionally, PDGF-BB interacts with XLKD1, LRP1, and SORL1, further emphasizing its versatile involvement in cellular regulation.

Measured by its binding ability in a functional ELISA. Immobilized Human PDGFRB at 2 μg/mL (100 μl/well) can bind Mouse PDGF-B His, the EC₅₀ of Mouse PDGF-B His is 4-24 ng/mL.

Mouse

P. pastoris

N-His

AAH23427.1 (S82-T190)

18591  [NCBI]

Approximately 14.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of30 % CAN, 0.1 % TFA.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.