PDGF R alpha Protein, Human (HEK293, His)

PDGF R α is a tyrosine protein kinase and an important cell surface receptor for PDGFA, PDGFB and PDGFC, affecting embryonic development, cell proliferation, survival and chemotaxis. Its effects on cell behavior vary, promoting or inhibiting proliferation and migration depending on environmental cues. PDGF R alpha Protein, Human (HEK293, His) is the recombinant human-derived PDGF R alpha protein, expressed by HEK293 , with C-6*His labeled tag.

PDGF R alpha, a tyrosine-protein kinase, assumes a pivotal role as a cell-surface receptor for PDGFA, PDGFB, and PDGFC, exerting significant influence over embryonic development, cell proliferation, survival, and chemotaxis. Its impact on cell behavior varies, as it can either promote or inhibit cell proliferation and migration based on contextual cues. The receptor's indispensable role extends to the differentiation of bone marrow-derived mesenchymal stem cells and is critical for normal skeletal development, embryonic cephalic closure, and the formation of the gastrointestinal mucosa. Moreover, PDGF R alpha contributes to the recruitment of mesenchymal cells and the development of intestinal villi. It plays a crucial role in wound healing by influencing cell migration and chemotaxis. PDGF R alpha's engagement with its ligands activates diverse signaling cascades, modulated by ligand specificity and influenced by heterodimer formation between PDGFRA and PDGFRB. The receptor phosphorylates key targets such as PIK3R1, PLCG1, and PTPN11, triggering downstream events like the AKT1 signaling pathway activation and induction of MAP kinase and STAT family members. The dynamic regulation of PDGF R alpha signaling involves the interplay of protein phosphatases and rapid internalization of the activated receptor to finely tune its cellular responses.

Human

HEK293

C-6*His

P16234-1 (Q24-E524)

5156  [NCBI]

QLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHSRDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSE

Approximately 93 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.