PDK3 Protein, Mouse (His)

PDK3 is a metabolic regulator that inhibits pyruvate dehydrogenase through PDHA1 phosphorylation to fine-tune glucose metabolism and affect aerobic respiration. Phosphorylation of PDHA2 adds another layer of control. PDK3 Protein, Mouse (His) is the recombinant mouse-derived PDK3 protein, expressed by E. coli , with N-His labeled tag.

PDK3, a pivotal player in metabolic regulation, exerts its influence by inhibiting pyruvate dehydrogenase activity through the phosphorylation of the E1 subunit PDHA1, thereby finely tuning glucose metabolism and aerobic respiration. Its capacity to phosphorylate PDHA2 adds another layer of control to these critical cellular processes. Under conditions of prolonged fasting or in response to a high-fat diet, PDK3 orchestrates a metabolic shift, reducing glucose utilization and enhancing fat metabolism. Beyond its role in nutrient adaptation, PDK3 emerges as a key contributor to glucose homeostasis, playing a crucial part in maintaining normal blood glucose levels. Additionally, its involvement in the generation of reactive oxygen species adds a layer of complexity to its multifaceted functions.

Mouse

E. coli

N-His

Q922H2 (I131-S362)

236900  [NCBI]

IEYKEKFGFDPFISSNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPAHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPNKPIQVVYVPSHLFHMLFELFKNSMRATVELHEDKKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALS

Approximately 30.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.