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  4. Pepsinogen A4/PGA4 Protein, Human (HEK293, Fc)

Pepsinogen A4/PGA4 Protein, Human (HEK293, Fc)

Cat. No.: HY-P77135
Handling Instructions

Pepsinogen A4/PGA4 protein, known for broad substrate specificity, prefers cleaving phenylalanine and leucine bonds but also shows the ability to cleave various other bonds to varying extents. Pepsinogen A4/PGA4 Protein, Human (HEK293, Fc) is the recombinant human-derived Pepsinogen A4/PGA4 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.

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Description

Pepsinogen A4/PGA4 protein, known for broad substrate specificity, prefers cleaving phenylalanine and leucine bonds but also shows the ability to cleave various other bonds to varying extents. Pepsinogen A4/PGA4 Protein, Human (HEK293, Fc) is the recombinant human-derived Pepsinogen A4/PGA4 protein, expressed by HEK293 , with C-hFc labeled tag.
Background

Pepsinogen A4/PGA4 protein exhibits a notably broad substrate specificity, with a preference for cleaving bonds involving phenylalanine and leucine. Despite this preference, it demonstrates the ability to cleave many other types of bonds to varying extents.
Species

Human
Source

HEK293
Tag

C-hFc
Accession

P0DJD7/NP_001073276.1 (I16-A388)
Gene ID
Molecular Construction
N-term
PGA4 (I16-A388)
Accession # P0DJD7/NP_001073276.1
hFc
C-term
Synonyms
Pepsin A-4; Pepsinogen-4; PGA4
Molecular Weight

65-70 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Pepsinogen A4/PGA4 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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