PKLR Protein, Rat (P. pastoris, His)

The PKLR protein is a pyruvate kinase that plays a critical role in glycolysis, converting phosphoenolpyruvate to pyruvate and producing ATP.As a key enzyme, PKLR mediates the final steps of glycolysis, contributing to energy production.PKLR Protein, Rat (P.pastoris, His) is the recombinant rat-derived PKLR protein, expressed by P.pastoris , with N-6*His labeled tag.

PKLR Protein, identified as a pyruvate kinase, assumes a pivotal role in glycolysis by catalyzing the conversion of phosphoenolpyruvate to pyruvate, accompanied by the synthesis of ATP. As a key enzyme in this metabolic pathway, PKLR serves as a critical mediator of energy production by facilitating the final steps of glycolysis. The catalytic activity of PKLR contributes to the generation of pyruvate, a central metabolite with implications in various cellular processes, including energy metabolism and the tricarboxylic acid (TCA) cycle. The significance of PKLR in glycolytic flux underscores its fundamental role in cellular energy homeostasis and highlights its potential as a target for therapeutic interventions aimed at modulating metabolic pathways.

Rat

P. pastoris

N-6*His

P12928 (M1-S574)

24651  [NCBI]

MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS

64.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.