Plasma kallikrein/KLKB1 Protein, Mouse (HEK293, His)

Plasma kallikrein/KLKB1 enzymatically cleaves Lys-Arg and Arg-Ser bonds, triggering factor XII activation upon binding to a negatively charged surface.It liberates bradykinin from HMW kininogen and is implicated in the renin-angiotensin system, potentially converting prorenin into renin.This underscores the diverse and essential functions of Plasma kallikrein/KLKB1 in various physiological processes.Plasma kallikrein/KLKB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Plasma kallikrein/KLKB1 protein, expressed by HEK293 , with C-6*His labeled tag.

The Plasma kallikrein/KLKB1 protein demonstrates enzymatic activity by selectively cleaving Lys-Arg and Arg-Ser bonds. Upon binding to a negatively charged surface, it initiates a reciprocal activation of factor XII. Additionally, the protein plays a crucial role in releasing bradykinin from HMW kininogen. Intriguingly, it is implicated in the renin-angiotensin system, potentially contributing to the conversion of prorenin into renin. This intricate interplay underscores the diverse and essential functions of the Plasma kallikrein/KLKB1 protein in various physiological processes.

Measured by its ability to cleave 10 μM fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >300 pmol/min/µg.

Mouse

HEK293

C-6*His

P26262 (G20-A638)

16621

75-85 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM NaAc, 150 mM NaCl, pH 5.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.