PLY Protein, Streptococcus pneumoniae serotype 4 (N-His)

The PLY protein, a cholesterol-dependent toxin, induces cytolysis by forming pores in host membranes with a significant conformational change and oligomeric pore complex formation. Cholesterol is crucial for binding, insertion, and pore formation. PLY Protein, Streptococcus pneumoniae serotype 4 (N-His) is the recombinant PLY protein, expressed by E. coli , with N-His labeled tag. The total length of PLY Protein, Streptococcus pneumoniae serotype 4 (N-His) is 470 a.a., with molecular weight of ~55 kDa.

The PLY protein operates as a cholesterol-dependent toxin, inducing cytolysis by forming pores in host membranes containing cholesterol. Following its binding to target membranes, the protein undergoes a significant conformational change, facilitating its insertion into the host membrane and the subsequent formation of an oligomeric pore complex. The presence of cholesterol is crucial for various stages of this process, including binding to host membranes, membrane insertion, and pore formation, with cholesterol binding mediated by a Thr-Leu pair in the C-terminus. Notably, PLY protein exhibits reversibility and can be inactivated by oxidation. The resulting homooligomeric pore complex, comprising 35 to 50 subunits, is formed upon insertion into the host membrane, contributing to the protein's cytolytic activity.

Others

E. coli

N-His

P0C2J9 (A2-D471)

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ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Approximately 55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration, please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.