PPID Protein, Human (His)

PPID protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of prolyl imide peptide bonds and contributes to protein folding. It serves as a co-chaperone in the HSP90 complex, contributing to dynamic regulation and may favor the estrogen receptor complex. PPID Protein, Human (His) is the recombinant human-derived PPID protein, expressed by E. coli , with N-6*His, C-6*His labeled tag. The total length of PPID Protein, Human (His) is 370 a.a., with molecular weight of ~43.85 kDa.

PPID Protein serves as a peptidyl-prolyl cis-trans isomerase (PPIase), catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and potentially aiding in protein folding processes. Proposed to act as a co-chaperone in HSP90 complexes, particularly in unligated steroid receptors heterocomplexes, PPID contributes to the dynamic regulation of HSP90-co-chaperone-receptor complexes. This involvement suggests a role in tissue-specific control of receptor activity, with a potential preference for estrogen receptor complexes over glucocorticoid receptor complexes. Moreover, PPID may participate in the cytoplasmic dynein-dependent movement of receptors from the cytoplasm to the nucleus. Notably, it is implicated in regulating MYB by inhibiting its DNA-binding activity and plays a role in AHR signaling by promoting the formation of the AHR:ARNT dimer, independently of HSP90 but requiring chaperone activity. Additionally, PPID is involved in the regulation of UV radiation-induced apoptosis and promotes cell viability in anaplastic lymphoma kinase-positive anaplastic large-cell lymphoma (ALK+ ALCL) cell lines. In the context of microbial infection, PPID may be associated with hepatitis C virus (HCV) replication and release.

Measured by its ability to convert the substrate, Suc-AAPF-pNA, from Cis to Trans formation. The specific activity is 904.66 pmol/min/μg, as measured under the described conditions.

Human

E. coli

N-6*His;C-6*His

Q08752 (M1-A370)

5481  [NCBI]

MSHPSPQAKPSNPSNPRVFFDVDIGGERVGRIVLELFADIVPKTAENFRALCTGEKGIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

Approximately 43.85 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.