PPM1A Protein, Human (His)

PPM1A is a multifunctional enzyme that negatively regulates multiple signaling pathways. It significantly attenuates TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export, and signal termination. PPM1A Protein, Human (His) is the recombinant human-derived PPM1A protein, expressed by E. coli , with C-6*His labeled tag.

PPM1A, an enzyme with broad specificity, functions as a negative regulator in various signaling pathways. It plays a pivotal role in the attenuation of TGF-beta signaling by dephosphorylating SMAD2 and SMAD3. This enzymatic action leads to the dissociation of SMAD2 and SMAD3 from SMAD4, their nuclear export, and ultimately, the termination of TGF-beta-mediated signaling. Additionally, PPM1A dephosphorylates PRKAA1 and PRKAA2, contributing to the modulation of cellular processes. Another significant aspect of its regulatory function involves the termination of TNF-alpha-mediated NF-kappa-B activation. In this context, PPM1A dephosphorylates and inactivates IKBKB/IKKB, contributing to the downregulation of NF-kappa-B signaling.

Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 1060.04 nmol/min/mg.

Human

E. coli

C-6*His

P35813-1 (G2-W382)

5494  [NCBI]

GAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW

Approximately 45.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.