1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. PRMT3 Protein, Human (His)

PRMT3 Protein, Human (His)

Cat. No.: HY-P76551
COA Handling Instructions

PRMT3 protein is a multifunctional protein arginine N-methyltransferase. As a type I methyltransferase, it catalyzes monomethylation and asymmetric dimethylation of arginine residues in target proteins. In addition to its methyltransferase role, PRMT3 may also regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. PRMT3 Protein, Human (His) is the recombinant human-derived PRMT3 protein, expressed by E. coli , with N-10*His labeled tag. The total length of PRMT3 Protein, Human (His) is 530 a.a., with molecular weight of ~65 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

PRMT3 protein is a multifunctional protein arginine N-methyltransferase. As a type I methyltransferase, it catalyzes monomethylation and asymmetric dimethylation of arginine residues in target proteins. In addition to its methyltransferase role, PRMT3 may also regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. PRMT3 Protein, Human (His) is the recombinant human-derived PRMT3 protein, expressed by E. coli , with N-10*His labeled tag. The total length of PRMT3 Protein, Human (His) is 530 a.a., with molecular weight of ~65 kDa.

Background

PRMT3 protein, a versatile protein-arginine N-methyltransferase, catalyzes the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, categorizing it as a type I methyltransferase. In addition to its role as a methyltransferase, PRMT3 is implicated in potentially regulating retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity, highlighting its involvement in modulating key cellular processes. The dual enzymatic activities of PRMT3 underscore its significance in the post-translational modification of proteins and suggest its potential impact on diverse biological pathways, particularly those related to retinoic acid metabolism and signaling.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-10*His

Accession

O60678-1 (C2-Q531)

Gene ID
Molecular Construction
N-term
10*His
PRMT3 (C2-Q531)
Accession # O60678
C-term
Synonyms
Protein arginine N-methyltransferase 3; PRMT3; HRMT1L3
AA Sequence

CSLASGATGGRGAVENEEDLPELSDSGDEAAWEDEDDADLPHGKQQTPCLFCNRLFTSAEETFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ

Molecular Weight

Approximately 63 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PRMT3 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PRMT3 Protein, Human (His)
Cat. No.:
HY-P76551
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