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  4. Prokineticin-1/EG-VEGF Protein, Human (sf9, His)

Prokineticin-1/EG-VEGF Protein, Human (sf9, His)

Cat. No.: HY-P74616
COA Handling Instructions

Prokineticin-1/EG-VEGF protein is a potent regulator of gastrointestinal smooth muscle contraction and affects intestinal motility. Its multifaceted effects extend to capillary endothelial cells, inducing proliferation, migration, and fenestration and are specific to certain cell types. Prokineticin-1/EG-VEGF Protein, Human (sf9, His) is the recombinant human-derived Prokineticin-1/EG-VEGF protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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50 μg $320 In-stock
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Description

Prokineticin-1/EG-VEGF protein is a potent regulator of gastrointestinal smooth muscle contraction and affects intestinal motility. Its multifaceted effects extend to capillary endothelial cells, inducing proliferation, migration, and fenestration and are specific to certain cell types. Prokineticin-1/EG-VEGF Protein, Human (sf9, His) is the recombinant human-derived Prokineticin-1/EG-VEGF protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

Prokineticin-1/EG-VEGF Protein stands out as a potent regulator of gastrointestinal (GI) smooth muscle contraction, highlighting its role in modulating gut motility. Beyond its influence on smooth muscle, this protein exhibits a multifaceted impact on capillary endothelial cells derived from endocrine glands, inducing proliferation, migration, and fenestration. Importantly, Prokineticin-1/EG-VEGF demonstrates specificity by having little or no effect on various other endothelial and non-endothelial cell types. Its role extends to the enteric neural crest cells, where it induces proliferation and differentiation. In neuroblastoma progression, this protein directly influences the proliferation and migration of neuroblastoma cells, implicating its involvement in tumor dynamics. Additionally, Prokineticin-1/EG-VEGF positively regulates PTGS2 expression and prostaglandin synthesis, suggesting a potential role in inflammatory responses. With implications in placentation and normal as well as pathological testis angiogenesis, Prokineticin-1/EG-VEGF emerges as a versatile signaling molecule with diverse cellular effects across various physiological and pathological contexts.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

P58294 (A20-F105)

Gene ID
Molecular Construction
N-term
EG-VEGF (A20-F105)
Accession # P58294
His
C-term
Synonyms
Prokineticin-1; EG-VEGF; Mambakine; PROK1
Molecular Weight

Approximately 15 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 0.02% Tween-80, 10% gly, 1 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Prokineticin-1/EG-VEGF Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Prokineticin-1/EG-VEGF Protein, Human (sf9, His)
Cat. No.:
HY-P74616
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