Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His)

Acid prostatic phosphatase (ACPP) is an enzyme that catalyzes the conversion of orthophosphate monoesters into alcohol and orthophosphate. ACPP is regulated by androgens and secreted by prostate epithelial cells. Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is the recombinant human-derived Prostatic acid phosphatase/ACPP protein, expressed by HEK293 , with C-His labeled tag. The total length of Prostatic acid phosphatase/ACPP Protein, Human (HEK293, His) is 350 a.a., with molecular weight of 43-55 kDa.

Prostatic acid phosphatase (ACPP) is an enzyme responsible for catalyzing the conversion of orthophosphoric monoester to alcohol and orthophosphate. Synthesized under androgen regulation, ACPP is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant has been identified, encoding a longer isoform with a transmembrane domain that localizes to the plasma membrane-endosomal-lysosomal pathway. The expression of ACPP is notably confined to the prostate, emphasizing its role as a marker associated with this specific tissue.

1. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >100,000 pmol/min/μg.
2. Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). which can be measured by absorbance at 410 nm. The specific activity is 4.264×10⁷ pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-His

NP_001127666.1 (K33-K382)

55  [NCBI]

MRAAPLLLARAASLSLGFLFLLFFWLDRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQVLK

43-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of 20 mM PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.