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  4. Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His)

Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His)

Cat. No.: HY-P700619
Handling Instructions

Prostatic acid phosphatase (ACPP) operates as a versatile tyrosine phosphatase under acidic conditions (pH 4-6), dephosphorylating various substrates, including orthophosphate monoesters. With lipid phosphatase activity, ACPP inactivates lysophosphatidic acid in seminal plasma. In prostate cancer, it acts as a tumor suppressor by dephosphorylating ERBB2, deactivating MAPK signaling, and controlling cancer-related processes. ACPP also exhibits ecto-5'-nucleotidase activity in dorsal root ganglion neurons, suggesting a role in pain modulation by generating adenosine from AMP. Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His) is the recombinant human-derived Prostatic acid Phosphatase/ACPP protein, expressed by HEK293, with C-His, GFP labeled tag. The total length of Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His) is 350 a.a., with molecular weight of 70.6 kDa.

For research use only. We do not sell to patients.

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Description

Prostatic acid phosphatase (ACPP) operates as a versatile tyrosine phosphatase under acidic conditions (pH 4-6), dephosphorylating various substrates, including orthophosphate monoesters. With lipid phosphatase activity, ACPP inactivates lysophosphatidic acid in seminal plasma. In prostate cancer, it acts as a tumor suppressor by dephosphorylating ERBB2, deactivating MAPK signaling, and controlling cancer-related processes. ACPP also exhibits ecto-5'-nucleotidase activity in dorsal root ganglion neurons, suggesting a role in pain modulation by generating adenosine from AMP. Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His) is the recombinant human-derived Prostatic acid Phosphatase/ACPP protein, expressed by HEK293, with C-His, GFP labeled tag. The total length of Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His) is 350 a.a., with molecular weight of 70.6 kDa.

Background

Prostatic acid phosphatase (ACPP) is a non-specific tyrosine phosphatase that operates under acidic conditions (pH 4-6), demonstrating its versatility in dephosphorylating a diverse array of substrates, including alkyl, aryl, and acyl orthophosphate monoesters, as well as phosphorylated proteins. Notably, ACPP exhibits lipid phosphatase activity, contributing to the inactivation of lysophosphatidic acid in seminal plasma. Functioning as a tumor suppressor in prostate cancer, ACPP plays a crucial role in dephosphorylating ERBB2 and deactivating MAPK-mediated signaling, thereby exerting control over cellular processes implicated in cancer progression. Beyond its tyrosine phosphatase activity, ACPP showcases ecto-5'-nucleotidase activity in dorsal root ganglion neurons, generating adenosine from AMP. This additional function suggests a role in pain modulation, where adenosine acts as a pain suppressor, emphasizing the multifaceted nature of ACPP in cellular processes.

Species

Human

Source

HEK293

Tag

C-His;GFP

Accession

P15309 (K33-K382)

Gene ID

55  [NCBI]

Molecular Construction
N-term
ACPP (K33-K382)
Accession # P15309
GFP-His
C-term
Synonyms
ACP-3; ACPP; Prostatic Acid Phosphatase; PAPf39; PAP
AA Sequence

KELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQVLK

Molecular Weight

70.6 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Prostatic acid Phosphatase/ACPP Protein, Human (HEK293, GFP-His)
Cat. No.:
HY-P700619
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