PTPN12 Protein, Human

The PTPN12 protein is a multifunctional enzyme that can dephosphorylate a variety of proteins and regulate cell signaling cascades. According to literature, PTPN12 selectively targets tyrosine kinases such as ERBB2 and PTK2B/PYK2 to control related pathways. PTPN12 Protein, Human is the recombinant human-derived PTPN12 protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes), , , , mutation. The total length of PTPN12 Protein, Human is 355 a.a., with molecular weight of ~41 kDa.

PTPN12 Protein, the subject, functions as a versatile enzyme capable of dephosphorylating a range of proteins, thereby playing a crucial role in the regulation of various cellular signaling cascades, as documented in the literature. Among its substrates, PTPN12 selectively dephosphorylates cellular tyrosine kinases, including ERBB2 and PTK2B/PYK2, exerting regulatory control over signaling pathways involving these kinases. The specificity of PTPN12 is highlighted by its ability to dephosphorylate ERBB2 at specific tyrosine residues, such as 'Tyr-1112,' 'Tyr-1196,' and/or 'Tyr-1248.' The intricate dephosphorylation activity of PTPN12 underscores its significance in modulating key signaling events and fine-tuning cellular responses mediated by tyrosine kinase pathways.

Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site.The specific activity is >20 µmol/min/mg.

Human

Sf9 insect cells

Tag Free

Q05209 (N-G&P, M1-Q355)

5782  [NCBI]

Approximately 41 kDa

Greater than 85% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.