PVRIG Protein, Human (HEK293, Fc)

The PVRIG protein functions as a cell surface receptor for NECTIN2 and may serve as a co-inhibitory receptor capable of inhibiting T-cell receptor-mediated signaling. Upon interaction with NECTIN2, PVRIG inhibits T cell proliferation, suggesting its potential role in regulating immune responses. PVRIG Protein, Human (HEK293, Fc) is the recombinant human-derived PVRIG protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PVRIG Protein, Human (HEK293, Fc) is 131 a.a., with molecular weight of 45-50 kDa.

PVRIG (PVR-related immunoglobulin domain-containing) protein is identified as a cell surface receptor for NECTIN2, functioning as a potential coinhibitory receptor that dampens T-cell receptor-mediated signals. Upon interacting with NECTIN2, PVRIG exerts its inhibitory effect by suppressing T-cell proliferation. Notably, PVRIG engages in a competitive binding scenario with CD226 for NECTIN2, implying a regulatory role in immune responses where it competes with an activating receptor. This competitive interaction with CD226 underscores PVRIG's involvement in modulating the delicate balance between activating and inhibitory signals during T-cell activation.

10 μg/mL (100 μL/well) of immobilized Human PVRIG-Fc can bind Human Nectin-2-His with an ED50 value of 2.914 μg/mL, corresponding to an affinity constant of 168.5 nM.

Human

HEK293

C-hFc

Q6DKI7 (T41-D171)

79037  [NCBI]

TPEVWVQVRMEATELSSFTIRCGFLGSGSISLVTVSWGGPNGAGGTTLAVLHPERGIRQWAPARQARWETQSSISLILEGSGASSPCANTTFCCKFASFPEGSWEACGSLPPSSDPGLSAPPTPAPILRAD

45-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.