QPCT Protein, Human (sf9, His)

QPCT Protein synthesizes pyroglutamyl peptides, favoring N-terminal glutaminyl residue over adjacent acidic and tryptophan residues. It catalyzes N-terminal pyroglutamate formation, particularly [Glu-3]-amyloid-beta in APP peptides. In lab settings, it aids pyroglutamate formation in truncated amyloid-beta peptides. QPCT Protein may contribute to N-terminal pyroglutamate formation in peptides linked to amyloid-related plaque development. QPCT Protein, Human (sf9, His) is the recombinant human-derived QPCT protein, expressed by Sf9 insect cells , with N-His labeled tag.

QPCT Protein is responsible for the synthesis of pyroglutamyl peptides, displaying a preference against adjacent acidic and tryptophan residues to the N-terminal glutaminyl residue, while showing minimal impact on chain length beyond the second residue. It also catalyzes the formation of N-terminal pyroglutamate. In laboratory settings, it facilitates pyroglutamate formation in truncated forms of APP amyloid-beta peptides, specifically [Glu-3]-amyloid-beta. QPCT Protein might also play a role in the N-terminal pyroglutamate formation of various peptides associated with amyloid-related plaque formation.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

Q16769-1 (A33-L361)

25797  [NCBI]

Approximately 38 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.