RAB7A Protein, Rat (N-His)

RAB7A is a dynamic small GTPase that coordinates endolysosomal trafficking by cycling between a GTP-bound state and a GDP-bound state. In its active state, RAB7A interacts with various effectors, affecting endosomal maturation, microtubule directed migration, and endosome-lysosomal trafficking. RAB7A Protein, Rat (N-His) is the recombinant rat-derived RAB7A, expressed by E. coli , with N-His labeled tag. ,

RAB7A, a small GTPase, undergoes dynamic cycling between its active GTP-bound and inactive GDP-bound states, exerting multifaceted control over endo-lysosomal trafficking. In its active state, RAB7A engages with various effector proteins, influencing early-to-late endosomal maturation, microtubule-directed endosomal migration, and endosome-lysosome transport through intricate protein-protein interactions. Beyond its central role in endosomal traffic, RAB7A significantly impacts diverse cellular and physiological processes, including growth-factor-mediated cell signaling, nutrient-transporter-mediated nutrient uptake, neurotrophin transport in neuronal axons, and lipid metabolism. Moreover, it participates in specialized endosomal membrane trafficking events such as melanosome maturation, pathogen-induced phagosomes, and autophagosomes. RAB7A is pivotal in the maturation, acidification, and fusion of phagosomes with lysosomes, crucial for handling microbial pathogens. Its involvement extends to microbial pathogen infection and survival, influencing the life cycles of various viruses. Additionally, RAB7A collaborates with RAC1 to regulate the formation of ruffled borders in osteoclasts and plays roles in neurite outgrowth signaling, EGF-EGFR complex trafficking, ADRB2-stimulated lipolysis through lipophagy, exosomal release of specific proteins, cell surface expression of ACE2, and potentially in PRPH neuronal intermediate filament assembly.

Rat

E. coli

N-His

P09527 (M1-C207)

29448

MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNERAKASAESCSC

Approximately 23-25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.