RBKS Protein, Human (His)

RBKS protein plays a central role in cellular metabolism by catalyzing the phosphorylation of ribose at the O-5 site, a process that is dependent on ATP and magnesium. This enzymatic activity leads to the formation of D-ribose-5-phosphate, a versatile precursor that can be used in various biosynthetic pathways. RBKS Protein, Human (His) is the recombinant human-derived RBKS protein, expressed by E. coli , with N-6*His labeled tag.

The RBKS protein plays a vital role in cellular metabolism by catalyzing the phosphorylation of ribose at O-5, a reaction that requires ATP and magnesium. This enzymatic activity leads to the formation of D-ribose-5-phosphate, a crucial intermediate that can be utilized for the synthesis of nucleotides, including purines and pyrimidines, as well as histidine and tryptophan. Additionally, D-ribose-5-phosphate serves as a key component in the pentose phosphate pathway, contributing to the generation of reducing equivalents and nucleotide precursors. The versatility of RBKS in directing ribose-5-phosphate towards various biosynthetic pathways underscores its importance in coordinating cellular processes essential for nucleotide and energy metabolism.

Human

E. coli

N-6*His

Q9H477-1 (M1-F322)

64080  [NCBI]

MAASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF

Approximately 37 kDa

• 
  Greater than 85 % as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of sterile 50mM Tris-HCL, 300mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.