Ribonuclease R (rnr) protein, E.coli (His)

Ribonuclease R (RNase R) is a 3'-5' exoribonuclease crucial for structured RNA maturation, degrading rRNAs, tRNAs, and SsrA/tmRNA. It modulates ompA mRNA stability in the stationary phase, exhibiting processive activity and helicase function. RNase R, along with PNPase, ensures rRNA quality during steady-state growth and contributes to virulence gene expression in E. coli. Ribonuclease R (rnr) protein, E.coli (His) is the recombinant E. coli-derived Ribonuclease R, expressed by E. coli , with N-6*His labeled tag. The total length of Ribonuclease R (rnr) protein, E.coli (His) is 813 a.a., with molecular weight of ~90 kDa.

Ribonuclease R (RNase R) is a 3'-5' exoribonuclease that plays a crucial role in the maturation of structured RNAs, including rRNAs, tRNAs, and SsrA/tmRNA. It acts by releasing 5'-nucleoside monophosphates during the degradation process. Additionally, in the stationary phase, RNase R is involved in post-transcriptional regulation, specifically in the modulation of ompA mRNA stability. It exhibits processive activity, shortening RNA molecules to di- and trinucleotides. Interestingly, RNase R also possesses helicase activity, which is distinct from its RNase function. During starvation, RNases 2 and R (rnb and RNase R) contribute to the degradation of rRNA. However, under normal growth conditions, RNase R, along with PNPase, plays a more significant role in quality control of rRNA. These enzymes work together to ensure proper functioning and integrity of rRNA during steady-state growth. Additionally, RNase R is required for the expression of virulence genes in enteroinvasive strains of E. coli.

Measured by its ability to digest RNA, it reacted at 37°C for 30 minutes under a certain reaction system, and was identified by agar-gel electrophoresis. The optimal reaction concentration measured under the above conditions was 120 μg/mL-240 μg/mL.

E.coli

E. coli

N-6*His

P21499 (M1-E813)

948692

MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVELHIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVEGRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEAGVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNMGTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDARDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLPEVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQGDQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQTQRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLELPGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHFTSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRADEATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAATKAKRAAKKKVAE

Approximately 90 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% TX-100, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.