RPN2/Ribophorin II Protein, Human (HEK293, His)

RPN2/riboprotein II is a key subunit of the oligosaccharyltransferase (OST) complex, which catalyzes the initial glycan transfer during co-translational protein N-glycosylation. This critical step occurs in the nascent polypeptide chain and involves the transfer of defined glycans from polyhydroxypyrophosphate to asparagine residues in the Asn-X-Ser/Thr motif. RPN2/Ribophorin II Protein, Human (HEK293, His) is the recombinant human-derived RPN2/Ribophorin II protein, expressed by HEK293 , with C-His labeled tag. The total length of RPN2/Ribophorin II Protein, Human (HEK293, His) is 518 a.a., with molecular weight of ~60 kDa.

RPN2/Ribophorin II, a subunit of the oligosaccharyl transferase (OST) complex, plays a crucial role in catalyzing the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. This pivotal step marks the initiation of protein N-glycosylation, a cotranslational process occurring during protein synthesis. The OST complex, in which RPN2/Ribophorin II is an integral component, associates with the Sec61 complex at the translocon complex, facilitating the translocation of proteins across the endoplasmic reticulum (ER) membrane. The synergistic presence of all subunits within the OST complex is essential for achieving maximal enzyme activity. In essence, RPN2/Ribophorin II's involvement in protein modification, specifically protein glycosylation, underscores its fundamental role in the initial stages of N-glycosylation, contributing to the structural and functional diversity of glycoproteins in the cell.

Human

HEK293

C-His

P04844/NP_002942.2 (L23-V540)

6185  [NCBI]

LTPTHYLTKHDVERLKASLDRPFTNLESAFYSIVGLSSLGAQVPDAKKACTYIRSNLDPSNVDSLFYAAQASQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTARLSKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEEEAPSTVLSQNLFTPKQEIQHLFREPEKRPPTV

Approximately 60 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.