SETD7 Protein, Human (His)

The SETD7 protein is a histone methyltransferase that specifically monomethylates H3K4, a mark associated with transcriptional activation. Its role in gene activation extends to the collagenase and insulin genes, specifically at the insulin promoter when IPF1/PDX-1 is recruited. SETD7 Protein, Human (His) is the recombinant human-derived SETD7 protein, expressed by E. coli , with N-His labeled tag. The total length of SETD7 Protein, Human (His) is 365 a.a., with molecular weight of 47-50 kDa.

SETD7 Protein, a histone methyltransferase, demonstrates specificity in monomethylating 'Lys-4' of histone H3. This methylation event serves as a distinctive mark associated with epigenetic transcriptional activation. SETD7 plays a pivotal role in the activation of transcription for genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, it leads to the activation of transcription. Beyond histones, SETD7 exhibits methyltransferase activity toward non-histone proteins, including CGAS, p53/TP53, TAF10, and possibly TAF7, recognizing and binding the [KR]-[STA]-K motif in substrate proteins. Monomethylation of 'Lys-189' of TAF10 enhances its affinity for RNA polymerase II. Moreover, SETD7 monomethylates 'Lys-372' of p53/TP53, stabilizing the protein and amplifying p53/TP53-mediated transcriptional activation. Additionally, it monomethylates 'Lys-491' of CGAS, facilitating interaction between SGF29 and CGAS (By similarity).

Human

E. coli

N-His

Q8WTS6/NP_085151.1 (D2-K366)

80854  [NCBI]

DSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

47-50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.