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SFRP2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P74553
COA Handling Instructions

SFRP2 protein has multiple functions such as Wnt protein binding and receptor ligand activity, and regulates gene expression and signal transduction.It functions upstream in animal organ development, signal transduction, and regulation of endopeptidase activity.SFRP2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived SFRP2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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5 μg $125 In-stock
10 μg $213 In-stock
50 μg $595 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

SFRP2 protein has multiple functions such as Wnt protein binding and receptor ligand activity, and regulates gene expression and signal transduction.It functions upstream in animal organ development, signal transduction, and regulation of endopeptidase activity.SFRP2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived SFRP2 protein, expressed by HEK293 , with C-His labeled tag.

Background

SFRP2 protein exhibits diverse functions, including Wnt-protein binding activity, endopeptidase activator activity, and receptor ligand activity. It plays a crucial role in regulating gene expression, protein phosphorylation, and signal transduction. Operating upstream of various processes such as animal organ development, negative regulation of signal transduction, and the regulation of endopeptidase activity, SFRP2 is located in the extracellular space. The expression of SFRP2 spans multiple structures, including the alimentary system, brain, embryo mesenchyme, eye, and genitourinary system. The biased expression in specific tissues, such as the limb at embryonic day 14.5 and the adult mammary gland, suggests its involvement in tissue-specific developmental and regulatory processes.

Biological Activity

Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC50 value is 1.48 nM, under conditions in which Recombinant Mouse (rm) Frizzled-1 is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.

  • Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC50 value of SFRP2 is 1.48 nM, under conditions in which Recombinant Mouse (rm) Frizzled-1 is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
Species

Mouse

Source

HEK293

Tag

C-His

Accession

NP_033170.1 (L25-C295)

Gene ID
Molecular Construction
N-term
SFRP2 (L25-C295)
Accession # NP_033170.1
His
C-term
Synonyms
Secreted frizzled-related protein 2; FRP-2; SARP-1; FKSG12
AA Sequence

LFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKTKNEDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC

Molecular Weight

Approximately 31-36 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

SFRP2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SFRP2 Protein, Mouse (HEK293, His)
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