SHH Protein, Human (HEK293, His)

The SHH protein is cleaved into ShhN and ShhC fragments through autoproteolysis and cholesterol transferase activity. In the endoplasmic reticulum, ShhC is degraded, leaving behind lipidated ShhNp that is critical for developmental patterning. SHH Protein, Human (HEK293, His) is the recombinant human-derived SHH protein, expressed by HEK293 , with C-His labeled tag. The total length of SHH Protein, Human (HEK293, His) is 197 a.a., with molecular weight of ~24 kDa.

The sonic hedgehog (SHH) protein precursor undergoes autoproteolysis and cholesterol transferase activity in its C-terminal part, leading to the cleavage of the full-length protein into two fragments, ShhN and ShhC, with the covalent attachment of a cholesterol moiety to the C-terminus of the newly generated ShhN. These processes occur in the endoplasmic reticulum, where ShhC is subsequently degraded. The dually lipidated sonic hedgehog protein N-product (ShhNp) serves as a morphogen essential for diverse patterning events in development. ShhNp induces ventral cell fate in the neural tube and somites, participates in anterior-posterior axis patterning in the developing limb bud, and plays a crucial role in axon guidance. SHH binds to the patched (PTCH1) receptor, working in conjunction with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells and the ED₅₀ is typically 1-10 μg/mL.

Human

HEK293

C-His

Q15465 (M1-G197)

6469  [NCBI]

Approximately 24 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.