SHP-1 Protein, Human (His)

SHP-1 Protein modulates signaling from tyrosine-phosphorylated cell surface receptors, including KIT and EGFR. It enhances the inhibition of mast cell activation via the Lilrb4a receptor. The SH2 regions interact with cellular components to regulate its phosphatase activity. In collaboration with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. SHP-1 plays a crucial role in hematopoiesis. SHP-1 Protein, Human (His) is the recombinant human-derived SHP-1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of SHP-1 Protein, Human (His) is 299 a.a., with molecular weight of ~33.0 kDa.

SHP-1 protein acts as a critical modulator of signaling pathways initiated by tyrosine phosphorylated cell surface receptors, including KIT and the EGF receptor/EGFR. Additionally, it enhances the inhibition of mast cell activation mediated by the Lilrb4a receptor. The SH2 regions of SHP-1 may engage with other cellular components, influencing its phosphatase activity against interacting substrates. Notably, in collaboration with MTUS1, SHP-1 induces UBE2V2 expression in response to angiotensin II stimulation. Beyond these regulatory functions, SHP-1 plays a key role in hematopoiesis, further emphasizing its significance in the intricate network of cellular processes and signaling events.

Human

E. coli

C-6*His

AAH02523.1 (K243-I541)

5777  [NCBI]

KAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNI

Approximately 33.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 500 mM NaCl, 2 mM 2-Mercaptoethanol, 1 mM EDTA, 1 mM DTT, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.