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  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. CARKL Protein, Human (HEK293, His)

CARKL Protein, Human (HEK293, His)

Cat. No.: HY-P71308
Handling Instructions

CARKL Protein acts as a modulator of macrophage activation, controlling glucose metabolism and influencing the functional phenotype of macrophages. Its regulatory role underscores its significance in shaping cellular processes and contributes to understanding the molecular mechanisms governing immune responses and cellular homeostasis. CARKL Protein, Human (HEK293, His) is the recombinant human-derived CARKL protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.

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Description

CARKL Protein acts as a modulator of macrophage activation, controlling glucose metabolism and influencing the functional phenotype of macrophages. Its regulatory role underscores its significance in shaping cellular processes and contributes to understanding the molecular mechanisms governing immune responses and cellular homeostasis. CARKL Protein, Human (HEK293, His) is the recombinant human-derived CARKL protein, expressed by HEK293 , with C-6*His labeled tag.

Background

CARKL Protein functions as a modulator of macrophage activation, exerting control over glucose metabolism. Its regulatory role in the intricate interplay of cellular processes underscores its significance in orchestrating the activation state of macrophages. By influencing glucose metabolism, CARKL likely plays a pivotal role in shaping the functional phenotype of macrophages, contributing to the broader understanding of the molecular mechanisms governing immune responses and cellular homeostasis.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q9UHJ6 (M1-S478)

Gene ID
Molecular Construction
N-term
CARKL (M1-S478)
Accession # Q9UHJ6
6*His
C-term
Synonyms
Sedoheptulokinase; SHK; Carbohydrate kinase-like protein; SHPK; CARKL
AA Sequence

MAARPITLGIDLGTTSVKAALLRAAPDDPSGFAVLASCARAARAEAAVESAVAGPQGREQDVSRILQALHECLAALPRPQLRSVVGIGVSGQMHGVVFWKTGQGCEWTEGGITPVFEPRAVSHLVTWQDGRCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQVGVALGDLQASVYSCMAQRTDAVLNISTSVQLAASMPSGFQPAQTPDPTAPVAYFPYFNRTYLGVAASLNGGNVLATFVHMLVQWMADLGLEVEESTVYSRMIQAAVQQRDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPIQQLQEWGVERVMGSGSALSRNDVLKQEVQRAFPLPMSFGQDVDAAVGAALVMLRRHLNQKES

Molecular Weight

Approximately 53.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CARKL Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CARKL Protein, Human (HEK293, His)
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HY-P71308
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